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Sensing environment & community lifestyle in Pseudomonas aeruginosa

Laboratoire d'Ingénierie des Systèmes Macromoléculaires - Team C.Bordi

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Ali-Ahmad A, Fadel F, Sebban-Kreuzer C, Ba M, Pélissier GD, Bornet O, Guerlesquin F, Bourne Y, Bordi C, Vincent F. Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa. Sci. Rep. 2017 Sep 12;7(1):11262. [PubMed] PDF

Ali-Ahmad A, Bornet O, Fadel F, Bourne Y, Vincent F, Bordi C, Guerlesquin F, Sebban-Kreuzer C. NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1. Biomol NMR Assign. 2017 Apr;11(1):25-28. [PubMed] PDF

McCarthy RR, Mazon-Moya MJ, Moscoso JA, Hao Y, Lam JS, Bordi C, Mostowy S, Filloux A. Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion. Nat Microbiol. 2017 Mar 6;2:17027. [PubMed]


Kandalla PK, Sarrazin S, Molawi K, Berruyer C, Redelberger D, Favel A, Bordi C, de Bentzmann S, Sieweke MH. M-CSF improves protection against bacterial and fungal infections after hematopoietic stem/progenitor cell transplantation. J Exp Med. 2016 Oct 17;213(11):2269-2279. [PubMed] PDF

Chambonnier G, Roux L, Redelberger D, Fadel F, Filloux A, Sivaneson M, de Bentzmann S, Bordi C. The Hybrid Histidine Kinase LadS Forms a Multicomponent Signal Transduction System with the GacS/GacA Two Component System in Pseudomonas aeruginosa. PLOS Genetics 2016 May 13;12(5):e1006032. [PubMed] PDF

Mercy C, Ize B, Salcedo SP, de Bentzmann S, Bigot S. Functional Characterization of Pseudomonas Contact Dependent Growth Inhibition (CDI) Systems. PLoS One. 2016 Jan 25;11(1). [PubMed] PDF
Erratum in: PLoS One. 2016;11(2) [PubMed]


Lucchetti-Miganeh C, Redelberger D, Chambonnier G, Rechenmann F, Elsen S, Bordi C, Jeannot K, Attrée I, Plésiat P, de Bentzmann S. Pseudomonas aeruginosa Genome Evolution in Patients and under the Hospital Environment. Pathogens. 2014 Apr 10;3(2):309-40 [PubMed] PDF

Boukerb AM, Rousset A, Galanos N, Méar JB, Thépaut M, Grandjean T, Gillon E, Cecioni S, Abderrahmen C, Faure K, Redelberger D, Kipnis E, Dessein R, Havet S, Darblade B, Matthews SE, de Bentzmann S, Guéry B, Cournoyer B, Imberty A,Vidal S.Antiadhesive properties of glycoclusters against Pseudomonas aeruginosa lung infection.J Med Chem. 2014 Dec 26;57(24):10275-89. [PubMed]

Smadhi M, de Bentzmann S, Imberty A, Gingras M, Abderrahim R, Goekjian PG.Expeditive synthesis of trithiotriazine-cored glycoclusters and inhibition of Pseudomonas aeruginosa biofilm formation. Beilstein J Org Chem. 2014 Aug 25;10:1981-90. [PubMed] PDF

Eierhoff T, Bastian B, Thuenauer R, Madl J, Audfray A, Aigal S, Juillot S, Rydell GE, Müller S, de Bentzmann S, Imberty A,Fleck C, Römer W. A lipid zipper triggers bacterial invasion. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12895-900. [PubMed] PDF

Bordi C.Construction of a Pseudomonas aeruginosa genomic DNA library. Methods Mol Biol. 2014;1149:555-63. [PubMed]

Faure LM, Garvis S, de Bentzmann S, Bigot S. Characterization of a novel two-partner secretion system implicated in the virulence of Pseudomonas aeruginosa. Microbiology. 2014 Sep;160(Pt 9):1940-52. [PubMed] PDF

de Bentzmann S, Varrot A, Imberty A. Monitoring lectin interactions with carbohydrates. Methods Mol Biol. 2014;1149:403-14. [PubMed]

de Bentzmann S, Bordi C. Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays. Methods Mol Biol. 2014;1149:225-41. [PubMed]

Sall KM, Casabona MG, Bordi C, Huber P, de Bentzmann S, Attrée I, Elsen S. A gacS deletion in Pseudomonas aeruginosa cystic fibrosis isolate CHA shapes its virulence. PLoS One. 2014 Apr 29;9(4) [PubMed] PDF


Beaufort N, Corvazier E, Mlanaoindrou S, de Bentzmann S, Pidard D. Disruption of the endothelial barrier by proteases from the bacterial pathogen Pseudomonas aeruginosa: implication of matrilysis and receptor cleavage. PLoS One. 2013 Sep 19;8(9) [PubMed] PDF

Wallart A, Parguey V, de Bentzmann N, Bompoint C, Fournier I, Godin S, Lebars L, Maurer N, Porcheron S, Premel C, RenaudB, Boulanger F, Fort M, Marry E, Yakoub-Agha I; SFGM-TC.A report of the SFGM-TC on the interactions between national transplant coordination and local coordinators in allogeneic stem cell transplantation activity. Pathol Biol (Paris). 2013 Aug;61(4):144-6. [PubMed]

Faure LM, Llamas MA, Bastiaansen KC, de Bentzmann S, Bigot S. Phosphate starvation relayed by PhoB activates the expression of the Pseudomonas aeruginosa σvreI ECF factor and its target genes. Microbiology. 2013 Jul;159(Pt 7):1315-27. [PubMed] PDF


de Bentzmann S, Giraud C, Bernard CS, Calderon V, Ewald F, Plésiat P, Nguyen C, Grunwald D, Attree I, Jeannot K, Fauvarque MO, Bordi C. Unique biofilm signature, drug susceptibility and decreased virulence in Drosophila through the Pseudomonas aeruginosa two-component system PprAB. PLoS Pathog. 2012; 8(11) Erratum in: PLoS Pathog. 2012 Dec;8(12). [PubMed] PDF

Roux N, Spagnolo J, de Bentzmann S. Neglected but amazingly diverse type IVb pili. Res Microbiol. 2012 Nov-Dec;163(9-10):659-73 [PubMed]

Houot L, Fanni L., de Bentzmann S, Bordi C. (2012) A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa. Microbiology. 58:1964-71. [PubMed] PDF

Duque E, de la Torre J, Bernal P, Molina-Henares MA, Alaminos M, Espinosa-Urgel M, Roca M, Fernández M, de Bentzmann S, and Ramos JL. Identification of reciprocal adhesion genes in pathogenic and non-pathogenic Pseudomonas. Environ Microbiol. In press.Article first published online: 28 MAR 2012 | DOI: 10.1111/j.1462-2920.2012.02732.x [PubMed]

Spagnolo J, Bigot S, Denis Y, Bordi C, de Bentzmann S. Development of a genetic tool for activating chromosomal expression of cryptic or tightly regulated loci in Pseudomonas aeruginosa. Plasmid. 67(3):245-51 [PubMed]

Giraud C and de Bentzmann S. Inside the complex regulation of Pseudomonas aeruginosa chaperone usher systems. Env Microbiol. 14(8):1805-16 [PubMed] PDF


de Bentzmann S, Plésiat P. Pseudomonas aeruginosa : une virulence complexe. Revue Francophone des Laboratoires, 435 : 33-41

Bordi C, and de Bentzmann S. Hacking into bacterial biofilms : a new therapeutic challenge. Annals of Intensive Care. 1:19 [PubMed] PDF

Beaufort N, Corvazier E, Hervieu A, Choqueux C, Dussiot M, Louedec L, Cady A, de Bentzmann S, Michel JB, Pidard D. The Thermolysin-Like Metalloproteinase and Virulence Factor LasB from Pathogenic Pseudomonas aeruginosa Induces Anoikis of Human Vascular Cells. Cell Microbiol. 428:473-82 [PubMed] PDF

de Bentzmann S, Plésiat P. The Pseudomonas aeruginosa opportunistic pathogen and human infections. Env Microbiol. 2011; 13:1655-1665 [PubMed]

Sivaneson M, , Mikkelsen H, Ventre I, Bordi C, and Filloux A. Two-component regulatory systems in Pseudomonas aeruginosa : an intricate network mediating fimbrial and efflux pump gene expression. Mol Microbiol. In press. 2011 Dec 28. [PubMed] PDF

Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, and de Bentzmann S. The PprA-PprB two-component system activates CupE, the first non archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae. Env Microbiol 2011, 13: 666-683 [PubMed]


Vincent F, Round A, Reynaud A, Bordi C, Filloux A, Bourne Y. Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site. Environ Microbiol June 7 2010.

Bordi C, Lamy MC, Ventre I, Termine E, Hachani A, Fillet S, Roche B, Bleves S, Méjean V, Lazdunski A, Filloux A. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. Mol Microbiol 2010 Apr 1

Beaufort N, Seweryn P, de Bentzmann S, Kellermann J, Grebenchtchikov Nl, Schmitt M, Pidard D, Magdolen V. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa. Biochem J 2010; May 27;428(3):473-82.

Giraud C, Bernard C, Ruer S, de Bentzmann S. Biological “glue” and “Velcro”: Molecular tools for adhesion and biofilm formation in the hairy and gluey bug Pseudomonas aeruginosa". Env Microbiol 2010; Rep 2(3), 343–358.

Bernard CS, Brunet YR, Gueguen E, Cascales E. Nooks and crannies in type VI secretion regulation.J Bacteriol 2010; Aug;192(15):3850-60. Epub 2010 May 28. Review.

Bigot S and Marians KJ. DNA Chirality-dependent Stimulation of Topoisomerase IV Activity by the C-Terminal AAA+ domain of FtsK. Nucleic Acids Res. 2010; May;38(9):3031-40. Epub 2010 Jan 16.

Houot L, Chang S, Pickering BS, Absalon C, Watnick PI. The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways.J Bacteriol. 2010; 2Jun;192(12):3055-67. Epub 2010 Apr 16.

Houot L, Chang S, Absalon C, Watnick PI. Vibrio cholerae PTS control of carbohydrate transport, biofilm formation, and colonization of the germ-free mouse intestine. Infect. Immun. 2010; Apr;78(4):1482-94. Epub 2010 Feb 1.


Garvis S, Munder A, Ball G, de Bentzmann S, Wiehlmann L, Ewbank JJ, Tümmler B, Filloux A. Caenorhabditis elegans Semi-Automated Liquid Screen Reveals a Specialized Role for the Chemotaxis Gene cheB2 in Pseudomonas aeruginosa Virulence. Plos pathogens. 2009; Aug;5(8):e1000540. Epub 2009 Aug 7.

Bernard CS, Bordi C, Termine E, Filloux A, de Bentzmann S. Organization and PprB dependent control of the Pseudomonas aeruginosa tad locus involved in Flp pilus biology. J Bacteriol. 2009; 191:1961-73.

Bastonero S, Le Priol Y, Armand M, Bernard CS, Reynaud-Gaubert M, Olive D, Parzy D, de Bentzmann S, Capo C, Mege JL. New Microbicidal Functions of Tracheal Glands: Defective Anti-Infectious Response to Pseudomonas aeruginosa in Cystic Fibrosis. PLoS ONE. 2009; 4(4):e5357.

Chemani C, Imberty , de Bentzmann S, Pierre M, Wimmerová M, Guery BP , and Faure K. (2009) Role of LecA and LecB lectins in Pseudomonas aeruginosa induced lung injury and effect of carbohydrates ligands. Infect Immun. 2009; 77:2065-75.

Mikkelsen, H., Ball, G., Giraud, C., and Filloux, A. Expression of Pseudomonas aeruginosa cupD fimbrial genes is antagonistically controlled by RcsB and the EAL-containing PvrR response regulators. PLos One. 2009; 4: e6018.

Bonnet L, Bigot S, Chevalier F, Allemand JF and Barre FX. Asymmetric DNA requirements in Xer recombination activation by FtsK. Nucleic. Acids. Res. 2009; 37(7), 2371-80.


Bordi C, Butcher BG, Shi Q, Hachmann AB, Peters JE, Helmann JD. In vitro mutagenesis of Bacillus subtilis by using a modified Tn7 transposon with an outward-facing inducible promoter. Appl Environ Microbiol. 2008 Jun;74(11):3419-25.

Karauzum H*, Ferry T*, de Bentzmann S, Lina G, Bes M, Vandenesch F, Etienne J, Landmann R. Comparison of adhesion and virulence of two predominant hospital-acquired MRSA clones and clonal MSSA isolates. Infection and Immunity 2008 Nov;76(11):5133-8. Epub 2008 Sep 8.*: These authors contribute equally.

Aschtgen MS, Bernard CS, de Bentzmann S, Lloubès R, Cascales E. SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J. Bacteriol. 2008 Nov;190(22):7523-31. Epub 2008 Sep 19.

Ruer S, Ball G, Filloux A, de Bentzmann S. The “P-usher”, a novel protein transporter involved in fimbrial assembly and TpsA secretion . EMBO Journal. 2008; Oct 22;27(20):2669-80.


Ruer S, Stender S, Filloux A, de Bentzmann S. Assembly of fimbrial structures in Pseudomonas aeruginosa: functionality and specificity of chaperone-usher machineries. J. Bacteriol. 2007. May;189(9):3547-55.

Bernard CS, Sadasivam M, Shiomi D, Margolin W. An altered FtsA can compensate for the loss of essential cell division protein FtsN in Escherichia coli. Mol. Microbiol. 2007 Jun: 64 (5), 1289–1305.

Leduc D, Beaufort N, de Bentzmann S, Rousselle JC, Namane A, Chignard M, Pidard D. The Pseudomonas aeruginosa LasB metalloproteinase regulates the human urokinase-type plasminogen activator receptor through domain-specific endoproteolysis. Infect Immun. 2007 Aug;75(8):3848-58.

Bigot S, Sivanathan V, Possoz C, Barre FX, Cornet F. FtsK, a literate chromosome segregation machine. Mol. Microbiol. 2007; 64(6), 1434-41.


Ventre I, Goodman AL, Vallet-Gely I, Vasseur P, Soscia C, Molin S, Bleves S, Lazdunski A, Lory S, Filloux A. Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes. Proc. Natl. Acad. Sci. USA. 2006 Jan 3;103(1):171-6.

de Bentzmann S, Aurouze M, Ball G, Filloux A. FppA, a novel Pseudomonas aeruginosa prepilin peptidase involved in assembly of type IVb pili. J. Bacteriol. 2006 Jul;188(13):4851-60.

Filloux A, Ventre I. Two sensors to control bacterial life style: the choice between chronic or acute infection. Med. Sci. (Paris). 2006 Oct;22(10):811-4.

Lequette Y, Lee JH, Ledgham F, Lazdunski A, Greenberg EP. A distinct QscR regulon in the Pseudomonas aeruginosa quorum-sensing circuit. J. Bacteriol. 2006 May;188(9):3365-70.

Bigot S, Saleh OA, Cornet F, Allemand JF & Barre FX. Oriented loading of FtsK on KOPS. Nat Struct Mol Biol. 2006; 13(11), 1026-28.


Vasseur P, Vallet-Gely I, Soscia C, Genin S, Filloux A. The pel genes of the Pseudomonas aeruginosa PAK strain are involved at early and late stages of biofilm formation. Microbiology. 2005 Mar;151(Pt 3):985-97.

Kulasekara HD, Ventre I, Kulasekara BR, Lazdunski A, Filloux A, Lory S. A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes. Mol. Microbiol. 2005 Jan;55(2):368-80.

Bigot S, Saleh OA, Lesterlin C, Pages C, El Karoui M, Dennis C, Griegoriev M, Allemand JF, Barre FX & Cornet F. KOPS : DNA motifs that control E.coli chromosome segregation by orienting the FtsK translocase. Embo. J. 2005; 24(21), 3770-80.


Vallet I, Diggle SP, Stacey RE, Camara M, Ventre I, Lory S, Lazdunski A, Williams P, Filloux A. Biofilm formation in Pseudomonas aeruginosa: fimbrial cup gene clusters are controlled by the transcriptional regulator MvaT. J. Bacteriol. 2004 May;186(9):2880-90.

Lazdunski AM, Ventre I, Sturgis JN. Regulatory circuits and communication in Gram-negative bacteria. Nat. Rev. Microbiol. 2004 Jul;2(7):581-92.

Filloux A. The underlying mechanisms of type II protein secretion. Biochim. Biophys. Acta. 2004 Nov 11;1694(1-3):163-79.

de Bentzmann S, Tristan A, Etienne J, Brousse N, Vandenesch F, Lina G. Staphylococcus aureus isolates associated with necrotizing pneumonia bind to basement membrane type I and IV collagens and laminin. J. Infect. Dis. 2004 Oct 15;190(8):1506-15. Epub 2004 Sep 15.

Coraux C, Kileztky C, Polette M, Hinnrasky J, Zahm JM, Devillier P, de Bentzmann S, Puchelle E. Airway epithelial integrity is protected by a long-acting beta2-adrenergic receptor agonist. Am. J. Respir. Cell. Mol. Biol. 2004 May;30(5):605-12. Epub 2003 Oct 3.

Tinsley CR, Voulhoux R, Beretti JL, Tommassen J, Nassif X. Three homologues, including two membrane-bound proteins, of the disulfide oxidoreductase DsbA in Neisseria meningitidis: effects on bacterial growth and biogenesis of functional type IV pili. J. Biol. Chem. 2004 Jun 25;279(26):27078-87

Bordi C, Ansaldi M, Gon S, Jourlin-Castelli C, Iobbi-Nivol C, Méjean V. Genes regulated by TorR, the trimethylamine oxide response regulator of Shewanella oneidensis. J. Bacteriol. 2004 Jul;186(14):4502-9.

Barker AP, Vasil AI, Filloux A, Ball G, Wilderman PJ, Vasil ML. A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxis. Mol. Microbiol. 2004 Aug;53(4):1089-98.

Bragonzi A, Copreni E, de Bentzmann S, Ulrich M, Conese M. Airway epithelial cell-pathogen interactions. J. Cyst. Fibros. 2004 Aug;3 Suppl 2:197-201.

Bigot S, Corre J, Louarn JM, Cornet F & Barre FX. FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol. Microbiol. 2004; 54(4), 876-86.


Filloux A, Vallet I. Biofilm: set-up and organization of a bacterial community. Med. Sci. (Paris). 2003 Jan;19(1):77-83. [PubMed] PDF

Ledgham F, Soscia C, Chakrabarty A, Lazdunski A, Foglino M. Global regulation in Pseudomonas aeruginosa: the regulatory protein AlgR2 (AlgQ) acts as a modulator of quorum sensing. Res. Microbiol. 2003 Apr;154(3):207-13. [PubMed]

Ledgham F, Ventre I, Soscia C, Foglino M, Sturgis JN, Lazdunski A. Interactions of the quorum sensing regulator QscR: interaction with itself and the other regulators of Pseudomonas aeruginosa LasR and RhlR. Mol. Microbiol. 2003 Apr;48(1):199-210. [PubMed] PDF

Lazdunski A. Pseudomonas aeruginosa: a model of choice for the study of opportunistic pathogen. Ann. Fr. Anesth. Reanim. 2003 Jun;22(6):523-6. [PubMed]

Ventre I, Ledgham F, Prima V, Lazdunski A, Foglino M, Sturgis JN. Dimerization of the quorum sensing regulator RhlR: development of a method using EGFP fluorescence anisotropy. Mol. Microbiol. 2003 Apr;48(1):187-98. [PubMed] PDF

Bordi C, Iobbi-Nivol C, Méjean V, Patte JC. Effects of ISSo2 insertions in structural and regulatory genes of the trimethylamine oxide reductase of Shewanella oneidensis. J. Bacteriol. 2003 Mar;185(6):2042-5. [PubMed] PDF

Bordi C, Theraulaz L, Mejean V, Jourlin-Castelli C. Anticipating an alkaline stress through the Tor phosphorelay system in Escherichia coli. Mol. Microbiol. 2003 Apr;48(1):211-23. [PubMed] PDF

Kurz CL, Chauvet S, Andres E, Aurouze M, Vallet I, Michel GP, Uh M, Celli J, Filloux A, de Bentzmann S, Steinmetz I, Hoffmann JA, Finlay BB, Gorvel JP, Ferrandon D, Ewbank JJ. Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening. EMBO J. 2003 Apr 1;22(7):1451-60. [PubMed] PDF

Berthelot P, Attree I, Plesiat P, Chabert J, de Bentzmann S, Pozzetto B, Grattard F; Groupe d'Etudes des Septicémies à Pseudomonas aeruginosa. Genotypic and phenotypic analysis of type III secretion system in a cohort of Pseudomonas aeruginosa bacteremia isolates: evidence for a possible association between O serotypes and exo genes. J. Infect. Dis. 2003 Aug 15;188(4):512-8. Epub 2003 Jul 23. [PubMed] PDF