Transports macromoléculaires à travers l'enveloppe bactérienne



Cell Fractionation. Petiti M, Houot L, Duché D. Methods Mol Biol. 2017;1615:59-64.

Large Complexes: Cloning Strategy, Production, and Purification. Durand E, Lloubes R. Methods Mol Biol. 2017;1615:299-309.

Electrostatic interactions between the CTX phage minor coat protein and the bacterial host receptor TolA drive the pathogenic conversion of Vibrio cholerae. Houot L , Navarro R, Nouailler M, Duché D , Guerlesquin F, Lloubes R. J Biol Chem. 2017 Aug 18;292(33):13584-13598.

Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury. Bielaszewska M, Rüter C, Bauwens A, Greune L, Jarosch KA, Steil D, Zhang W, He X, Lloubes R , Fruth A, Kim KS, Schmidt MA, Dobrindt U, Mellmann A, Karch H. PLoS Pathog. 2017 Feb 3;13(2):e1006159.


Structural insight into the role of the Ton complex in energy transduction. Célia H , Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Cramer WA, Lloubes R and Buchanan SK. Nature. 2016 Oct 6;538(7623):60-65.

1H, 15N and 13C resonance assignments of the C-terminal domain of Vibrio cholerae TolA protein. Navarro R, Bornet O, Houot L , Lloubes R , Guerlesquin F Nouailler M. Biomol NMR Assign. 2016 Oct;10(2):311-3.


Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain. Kunsmann L, Rüter C, Bauwens A, Greune L, Glüder M, Kemper B, Fruth A, Wai SN, He X, Lloubes R , Schmidt MA, Dobrindt U, Mellmann A, Karch H, Bielaszewska M. Sci Rep. 2015 5: 13252.


Type VI secretion and bacteriophage tail tubes share a common assembly pathway. Brunet YR, Hénin J, Celia H , Cascales E . EMBO Rep. 15(3):315-21.


Non classical secretion systems. Lloubes R, Bernadac A, Houot L , Pommier S . Res Microbiol. 2013 Jul-Aug;164(6):655-63. Review.

Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation. Ymele-Leki P, Houot L , Watnick PI. Appl Environ Microbiol. 2013 Aug;79(15):4675-83.


A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa. Houot L , Fanni A, de Bentzmann S, Bordi C. Microbiology. 2012 Aug;158(Pt 8):1964-71.

Colicin M, a peptidoglycan lipid-II-degrading enzyme: potential use for antibacterial means? Touzé T, Barreteau H, El Ghachi M, Bouhss A, Barnéoud-Arnoulet A , Patin D, Sacco E, Blanot D, Arthur M, Duché D , Lloubès R , Mengin-Lecreulx D. Biochem Soc Trans. 2012 Dec 1;40(6):1522-7.

Energetics of colicin import revealed by genetic cross-complementation between the Tol and Ton systems. Lloubes R, Goemaere E, Zhang X, Cascales E, Duché D. Biochem Soc Trans.2012, 40(6):1480-5.

The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. Aschtgen MS, Zoued A, Lloubes R , Journet L, Cascales E. Microbiologyopen. 2012, 1(1):71-82.

Characterization of colicin M and its orthologs targeting bacterial cell wall peptidoglycan biosynthesis. Barreteau H, El Ghachi M, Barnéoud-Arnoulet A , Sacco E, Touzé T, Duché D , Gérard F, Brooks M, Patin D, Bouhss A, Blanot D, van Tilbeurgh H, Arthur M, Lloubes R , Mengin-Lecreulx D. Microb Drug Resist. 2012, 18(3):222-9.

Prominent role of the three Synechocystis PchR-like regulators in the defense against metal and oxidative stresses. Soni B, Houot L , Cassier-Chauvat C, Chauvat F. Open Journal of Biochemistry. 2012, 1, 1.


An epigenetic switch involving overlapping fur and DNA methylation optimizes expression of a type VI secretion gene cluster. Brunet YR, Bernard CS, Gavioli M , Lloubes R , Cascales E. PLoS Genet. 7(7):e1002205.

Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. Bernard CS, Brunet YR, Gavioli M , Lloubes R , Cascales E. J Bacteriol. 193(9):2158-67.

Mapping the interactions between Escherichia coli TolQ transmembrane segments. Zhang XY, Goemaere EL, Seddiki N, Célia, Gavioli M, Cascales E, Lloubes R. J Biol Chem. 286(13):11756-64.

High water solubility and fold in amphipols of proteins with large hydrophobic regions: oleosins and caleosin from seed lipid bodies. Gohon Y, Vindigni JD, Pallier A, Wien F, Celia H , Giuliani A, Tribet C, Chardot T, Briozzo P. Biochim Biophys Acta. 1808(3):706-16.


Channel domain of colicin A modifies the dimeric organization of its immunity protein. Zhang XY, Lloubes R, Duché D. J Biol Chem. 285(49):38053-61.

Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli. Barnéoud-Arnoulet A, Gavioli M, Lloubes R, Cascales E. J Bacteriol. 192(22):5934-42.

Toxicity of the colicin M catalytic domain exported to the periplasm is FkpA independent. Barnéoud-Arnoulet A , Barreteau H, Touzé T, Mengin-Lecreulx D, Lloubes R , Duché D. J Bacteriol 192(19):5212-9.

The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Aschtgen MS , Gavioli M , Dessen A, Lloubes R , Cascales E. Mol Microbiol. 275(4):886-99.

Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme. Barreteau H, Bouhss A, Gérard F, Duché D , Boussaid B, Blanot D, Lloubes R, Mengin-Lecreulx D, Touzé T. J Biol Chem. 285(16):12378-89.

The PTS regulates Vibrio cholerae biofilm formation through multiple independent pathways. Houot L. , Chang S., Absalon C., Pickering B., and Watnick PI. J. Bacteriol 192(12) :3055-67.

Vibrio cholerae PTS control of carbohydrate transport, biofilm formation, and colonization of the germ-free mouse intestine. Houot L. , Chang S., Absalon C., and Watnick P.I. Infect and Immunity 78(4):1482-94.

Determinants of the proton selectivity of the colicin A channel. S. Slatin, D. Duché and D. Baty. Biochemistry. 49; 4786-93.

Components of E. coli Energy-Transducing Complexes, ExbB and TolQ, Display Ion Channels. Zakharov S, Célia H , Datsenko K, Santamaria M, Whitelegge J, Lloubes R , Cramer W. Biophys J. vol. 98, issue 3, pp. 540a.


Mapping the interactions between escherichia coli tol subunits: rotation of the TolR transmembrane helix.
Zhang XY, Goemaere EL, Thomé R, Gavioli M, Cascales E, Lloubes R. J Biol Chem. 284(7):4275-82.
Immunity protein protects colicin E2 from OmpT protease.
Duché D, Issouf M, Lloubes R. J Biochem. 145(1):95-101.
FpvA bound to non-cognate pyoverdines: molecular basis of siderophore recognition by an iron transporter.
Greenwald J, Nader M, Celia H , Gruffaz C, Geoffroy V, Meyer JM, Schalk IJ, Pattus F. Mol Microbiol. 72(5):1246-59.


SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli.
Aschtgen MS, Bernard CS, De Bentzmann S, Lloubes R , Cascales E. J Bacteriol. 190(22):7523-31.
Visualization of interactions between siderophore transporters and the energizing protein TonB by native PAGE.
Choul-Li S, Adams H, Pattus F, Celia H. Electrophoresis. 29(6):1333-8.
The metal dependence of pyoverdine interactions with its outer membrane receptor FpvA.
Greenwald J, Zeder-Lutz G, Hagege A, Celia H , Pattus F. J Bacteriol. 190(20):6548-58.
Carbon nanotubes as templates for polymerized lipid assemblies.
Thauvin C, Rickling S, Schultz P, Célia H , Meunier S, Mioskowski C. Nat Nanotechnol. 3(12):743-8.


Movements of the TolR C-terminal domain depend on TolQR ionizable key residues and regulate activity of the Tol complex
Goemaere EL, Devert A, Lloubes R, Cascales E. J Biol Chem. 282(24):17749-57.
Colicin biology.
Cascales E, Buchanan SK, Duché D, Kleanthous C, Lloubes R, Postle K, Riley M, Slatin S, Cavard D. Microbiol Mol Biol Rev. 71(1):158-229.
Mutational analyses define helix organization and key residues of a bacterial membrane energy-transducing complex.
Goemaere EL, Cascales E, Lloubes R. J Mol Biol. 366(5):1424-36.
Colicin E2 is Still in Contact with its Receptor and Import Machinery when its Nuclease Domain enters the Cytoplasm.
D. Duché. J. Bacteriol. 189, 4217−22.


Release of immunity protein requires functional endonuclease colicin import machinery.
Duché D, Frenkian A, Prima V, Lloubes R. J Bacteriol. 188(24):8593-600.


Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motif.
Pommier S, Gavioli M, Cascales E, Lloubes R. J Bacteriol. 187(21):7526-34.
Solution structure of the E.coli TolA C-terminal domain reveals conformational changes upon binding to the phage g3p N-terminal domain.
Deprez C, Lloubes R , Gavioli M , Marion D, Guerlesquin F, Blanchard L. J Mol Biol. 346(4):1047-57.
Combining high-field EPR with site-directed spin labeling reveals unique information on proteins in action.
K. Möbius, A. Savitsky, C. Wegener, M. Plato, M. Fuchs, A Schnegg, A. A. Dubinskii, Y. A. Grishin, I. A. Grigorev, M Kühn, D. Duché , H Zimmermann and H.J. Steinhoff. Magn. Reson. Chem. 43, S4-S19.


Improved methods for producing outer membrane vesicles in Gram-negative bacteria.
Henry T, Pommier S , Journet L , Bernadac A, Gorvel JP, Lloubes R. Res Microbiol. 155(6):437-46.
Deletion analyses of the peptidoglycan-associated lipoprotein Pal reveals three independent binding sequences including a TolA box.
Cascales E, Lloubes R. Mol Microbiol. 51(3):873-85.
Gating movements of colicin A and colicin Ia are different.
S.L. Slatin, D. Duché , P.K. Kienker and D. Baty. J. Memb. Biol. 202, 73-83.
Spontaneous refolding of the pore-forming Colicin A toxin upon membrane association as studied by X-band and W-band high-field EPR spectroscopy.
Savitsky, M. Kühn, D. Duché , K. Möbtus and H-J. Steinhoff . J. Phys. Chem. B. 108 , 9541-9548.


Analysis of the Escherichia coli Tol-Pal and TonB systems by periplasmic production of Tol, TonB, colicin, or phage capsid soluble domains.
Bouveret E, Journet L , Walburger A, Cascales E , Bénédetti H, Lloubes R. Biochimie. 84(5-6):413-21.
Macromolecular import into Escherichia coli: the TolA C-terminal domain changes conformation when interacting with the colicin A toxin.
Deprez C, Blanchard L, Guerlesquin F, Gavioli M , Simorre JP, Lazdunski C, Marion D, Lloubes R. Biochemistry. 2002 Feb 26;41(8):2589-98.
Pal lipoprotein of Escherichia coli plays a major role in outer membrane integrity.
Cascales E , Bernadac A, Gavioli M , Lazzaroni JC, Lloubes R. J Bacteriol. 184(3):754-9.
Translocation of a functional protein by a voltage-dependent ion channel.
S.L. Slatin, A. Nardi, K. Jakes, D. Baty and D. Duché. Proc. Natl. Acad. Sci., USA, 99, 1286-91.
The pore-forming domain of colicin A fused to a signal peptide : a tool for studying pore-formation and inhibition.
D. Duché. Biochimie. 84, 455-464.


The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB.
Cascales E , Lloubes R , Sturgis JN. Mol Microbiol. 42(3):795-807.
The C-terminal half of the colicin A pore-forming domain is active in vivo and in vitro.
Nardi, S. L. Slatin, D. Baty and D. Duché. J. Mol. Biol. 307 : 1293-1303.
Colicin A immunity protein interacts with the hydrophobic helical hairpin of the colicin A channel domain in the Escherichia coli inner membrane.
Nardi, Y. Corda, D. Baty and D. Duché. J. Bacteriol. 183 ; 6721-6725.
Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm.
Journet L , Bouveret E, Rigal A, Lloubes R , Lazdunski C, Bénédetti H. Mol Microbiol 42(2):331-44.
The Tol-Pal proteins of the Escherichia coli cell envelope: an energized system required for outer membrane integrity?
Lloubes R, Cascales E , Walburger A, Bouveret E, Lazdunski C, Bernadac A, Journet L. Res Microbiol. 152(6):523-9.
Crystallization and preliminary crystallographic study of the periplasmic domain of the Escherichia coli TolR protein.
Abergel C, Journet L , Chenivesse S, Gavioli M , Lloubes R. Acta Crystallogr D Biol Crystallogr. 57(Pt 2):323-5.


Impairment of bile salt-dependent lipase secretion in human pancreatic tumoral SOJ-6 cells.
Caillol N, Pasqualini E, Lloubes R , Lombardo D. J Cell Biochem. 79(4):628-47.
Phosphorylation of the rat pancreatic bile-salt-dependent lipase by casein kinase II is essential for secretion.
Pasqualini E, Caillol N, Valette A, Lloubes R , Verine A, Lombardo D. Biochem J. 1;345 Pt 1:121-8.
roton motive force drives the interaction of the inner membrane TolA and outer membrane pal proteins in Escherichia coli.
Cascales E , Gavioli M , Sturgis JN, Lloubes R. Mol Microbiol. 38(4):904-15.
Colicin import into Escherichia coli cells requires the proximity of the inner and outer membranes and other factors.
Lazdunski C, Bouveret E, Rigal A, Journet L , Lloubes R , Bénédetti H. Int J Med Microbiol. 290(4-5):337-44.
Assignment of the 1H, 15N and 13C resonances of the C-terminal domain of the TolA protein of Escherichia coli, involved in cell envelope integrity.
Deprez C, Blanchard L, Simorre JP, Gavioli M , Guerlesquin F, Lazdunski C, Lloubes R , Marion D. J Biomol NMR. 18(2):179-80.